Uji Aktivitas Antioksidan pada Ekstrak Daun Jambu Biji (Psidium Guajava L) Menggunakan DPPH (2,2-Difenil-1-Pikrilhidrazil)

Authors

  • Dzakiyyah Syarifuddin Universitas Tadulako, Indonesia
  • Tahril Universitas Tadulako, Indonesia
  • Sitti Aminah Universitas Tadulako, Indonesia
  • Arwansyah Universitas Tadulako, Indonesia

DOI:

https://doi.org/10.37985/jer.v7i2.3577

Keywords:

Antioksidan Daun Jambu Biji DPPH Spektrofotometer UV-Vis

Abstract

Daun jambu biji Psidium guajava L. merupakan tanaman herbal yang mengandung senyawa bioaktif seperti flavonoid, tanin, saponin, dan alkaloid yang berpotensi sebagai antioksidan alami. Penelitian ini bertujuan untuk menganalisis dan membandingkan aktivitas antioksidan ekstrak daun jambu biji berdasarkan tingkat kepolaran pelarut. Ekstraksi dilakukan secara maserasi bertingkat menggunakan pelarut n-heksana, etil asetat, dan etanol. Aktivitas antioksidan dianalisis dengan metode DPPH menggunakan spektrofotometer UV-Vis dan dinyatakan dalam nilai IC50. Hasil penelitian menunjukkan bahwa ekstrak n-heksana memiliki nilai IC50 sebesar 169,913 ppm, ekstrak etil asetat sebesar 124,728 ppm, ekstrak etanol sebesar 47,620 ppm, dan vitamin C sebagai kontrol positif sebesar 24,347 ppm. Berdasarkan nilai IC50, ekstrak etanol memiliki aktivitas antioksidan kategori kuat, ekstrak etil asetat kategori sedang, sedangkan ekstrak n-heksana kategori lemah. Hasil ini menunjukkan bahwa pelarut etanol lebih efektif dalam mengekstraksi senyawa antioksidan pada daun jambu biji sehingga berpotensi dikembangkan sebagai sumber antioksidan alami dalam bidang pangan dan kesehatan.

Downloads

Download data is not yet available.

References

Ahyani, I. N., Mahbub, F., Kanalung, A. T. P., & Kusumaningtyas, F. A. (2025). Penetapan kadar flavonoid total dan uji aktivitas antioksidan ekstrak kulit coklat dengan metode DPPH dan FRAP. Majalah Farmaseutik, 21(2), 213. https://doi.org/10.22146/farmaseutik.v21i2.106730

Aprilianda Widyantari, N. K., Mia Ayuk Arthana, N. P., Agung Ariwijaya, D. G., Udayani, N. N. W., & Ayu Kusuma Wardani, I. G. A. (2024). Uji aktivitas antioksidan ekstrak etanol daun benalu jeruk (Dendrophthoe glabrescens (Blakely) Barlow) dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Indonesian Journal of Pharmaceutical Education, 4(1), 158–167. https://doi.org/10.37311/ijpe.v4i1.25409

Aqmal, M. N., Mulkiya, K., & Maharani, V. (2025). Pengujian aktivitas antioksidan ekstrak maserasi bertingkat biji buah alpukat (Persea americana Mill.) dengan metode DPPH. Bandung Conference Series: Pharmacy, 5(1), 253–259. https://doi.org/10.29313/bcsp.v5i1.18244

Azwanida, N. N. (2018). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants, 4(3), 196–201 https://doi.org/10.4172/2167-0412.1000323

Chairunnisa, S., Wartini, N. M., & Suhendra, L. (2019). Pengaruh suhu dan waktu maserasi terhadap karakteristik ekstrak daun bidara (Ziziphus mauritiana L.) sebagai sumber saponin: Effect of temperature and maseration time on characteristics of bidara leaf extract (Ziziphus mauritiana L.) as saponin source. Jurnal Rekayasa Dan Manajemen Agroindustri, 7(4), 551–560 https://doi.org/10.24843/jrma.2019.v07.i04.p07

Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y. H. (2019). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296–302. https://doi.org/10.1016/j.jfda.2013.11.001

Edy, U., Supriadi, Wahid, M., Diah, A., & Jura, M. R. (2023). Media eksakta. Available, 17(1), 46–51 https://doi.org/10.22487/me.v19i2

Hasan, H., Ain Thomas, N., Hiola, F., Nuzul Ramadhani, F., & Ibrahim, A. S. (2022). Skrining fitokimia dan uji aktivitas antioksidan kulit batang matoa (Pometia pinnata) dengan metode 1,1-diphenyl-2 picrylhidrazyl (DPPH). Indonesian Journal of Pharmaceutical Education, 2(1), 67–73. https://doi.org/10.37311/ijpe.v2i1.10995

Kumar, A. (2020). Antioxidant activity of guava leaf (Psidium guajava L.) extract against free radicals. Journal of Medicinal Plants Research, 14(5), 210–218 https://doi.org/10.9734/jocamr/2020/v10i230160

Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118–126. https://doi.org/10.4103/0973-7847.70902

Mastura, Safrida, C. L., & Nurhafidhah. (2023). Uji aktivitas antioksidan ekstrak etanol daun asoka (Ixora chinensis Lam.) dengan menggunakan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). KATALIS: Jurnal Penelitian Kimia Dan Pendidikan Kimia, 5(2), 18–26. https://doi.org/10.33059/katalis.v5i2.6969

Melinda, R., Sartika Daulay, A., Ridwanto, R., & Amin Nasution, M. (2024). Penetapan kadar vitamin C dan aktivitas antioksidan hasil perasan buah jambu biji kristal. Indonesian Journal of Pharmaceutical Education, 4(3), 438–449. https://doi.org/10.37311/ijpe.v4i3.28891

Nguyen, T. H. (2022). Flavonoid content and antioxidant activity of Psidium guajava leaf extract. International Journal of Phytopharmacy, 12(4), 145–153 https://doi.org/10.21275/sr22208170132

Ningsih, A. W., Arifin, J. F. N., Devitri, R. W., Septiarini, R. T., Zahara, E. S., Maulidya, V., Maulidya, Y. E., Rahmawati, E. E., Sabila, Z., Humaidah, Z., & Klau, I. C. S. (2025). Tinjauan literatur: Efektivitas maserasi sebagai metode ekstraksi fitokimia. OBAT: Jurnal Riset Ilmu Farmasi Dan Kesehatan, 3(6), 228–242. https://doi.org/10.61132/obat.v3i6.1927

Pham-Huy, L. A., He, H., & Pham-Huy, C. (2020). Free radicals, antioxidants in disease and health. International Journal of Biomedical Science, 4(2), 89–96 https://doi.org/10.5530/fra.2020.1

Potalangi, N. O., Palandi, R. R., Manoppo, A. J., Tewu, T. M., Kanter, J. W., & Sambou, C. N. (2024). Antioxidant activity test of ethanol extract of Helixanthera cylindrica (Jack) Danser mistletoe leaves on Moringa oleifera plants using the 2,2-diphenyl-1-picrylhydrazyl method. [Journal Name], [Volume(Issue)], 648–657 https://doi.org/10.2139/ssrn.4737728

Rahman, M. (2023). Quantitative evaluation of antioxidant activity using DPPH assay and IC50 determination. Journal of Applied Pharmaceutical Science, 13(1), 75–83 https://doi.org/10.1101/2023.01.03.522539

Ramdan, S. R. K. (2024). Pharmacy genius. [Journal Name], 3(1), 56–63 https://doi.org/10.56359/pharmgen.v3i01

Rossa, A., Daulay, A. S., Ridwanto, & Rahayu, Y. P. (2023). Antioxidant activity and toxicity of raru (Cotylelobium lanceolatum Craib) bark extract using the DPPH method and BSLT method. Journal of Pharmaceutical and Sciences, 1(1), 339–352 https://doi.org/10.36490/journal-jps.com.v6i5-si.436

Shahidi, F., & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. Journal of Functional Foods, 18, 820–897. https://doi.org/10.1016/j.jff.2015.06.018

Sies, H., Jones, D. P., & Roos, D. (2020). Oxidative stress. Annual Review of Biochemistry, 89, 715–748.

Suriawati, J., & Rachmawati, S. R. (2023). Aktivitas antioksidan ekstrak daun kelor metode DPPH dan FRAP sebagai sediaan obat dan makanan. Medical Sains: Jurnal Ilmiah Kefarmasian, 8(1), 253–262.

Tan, W. (2021). Sequential extraction using different polarity solvents for antioxidant analysis of medicinal plants. Journal of Herbal Science, 9(2), 88–97.

Tasiam, E., Farmasi, P., Matematika, F., & Alam, P. (2025). Antioxidant activity test of beluntas leaves (Pluchea indica Less.) using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Jurnal Farmasi Malahayati, 8(2), 272–281.

Yani, I. A., Suhaenah, A., & A. (2024). Uji aktivitas antioksidan ekstrak daun karet kebo (Ficus elastica) dengan menggunakan metode maserasi bertingkat. Makassar Pharmaceutical Science Journal, 2(3), 450–460. https://doi.org/10.33096/mpsj.v2i3.283

Zhang, Y., Li, X., Wang, Z., & Liu, H. (2021). Natural antioxidants from medicinal plants: Extraction and biological activity. Food Chemistry, 345, 128–140.

Downloads

Published

24-05-2026

Indexing Check

Google Scholar

How to Cite

Syarifuddin, D., Tahril, Aminah, S., & Arwansyah. (2026). Uji Aktivitas Antioksidan pada Ekstrak Daun Jambu Biji (Psidium Guajava L) Menggunakan DPPH (2,2-Difenil-1-Pikrilhidrazil). Journal of Education Research, 7(2), 549–560. https://doi.org/10.37985/jer.v7i2.3577

Issue

Vol. 7 No. 2 (2026)

Section

Articles

Categories

License

Copyright (c) 2026 Dzakiyyah Syarifuddin, Tahril, Sitti Aminah, Arwansyah

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International License that allows others to share the work with an acknowledgement of the works authorship and initial publication in this journal. 
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journals published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).